Genetic Diversity among Local Quail Using RAPD-DNA Marker

Abstract

The randomly amplified polymorphic DNA (RAPD) markers were used to detect the genetic variations and polymorphism among three different colors of local quail (desert, brown and white). Out of twenty random primers used, thirteen were able to amplify and showed bands. The total fragment number arrived 310 with size range from 250 to 2800 bp. Polymorphic fragments and unique bands in all samples were (34.36 and 2), respectively. However, OPA-04, OPS-01, OPB-01 and 10- MER loci have generated high polymorphic bands. The Nei’s gene diversity for overall lines averaged 0.1026.The higher distance found among brown female with both white and desert female (27.614), while the lowest distance (4.002) recorded between white and desert (male and female) quails. The overall dendrograms clustered the quail lines into two clusters. The 1st one consisted of white and desert, and the 2nd one included only brown. It was concluded that the white was closer to desert quail than to the brown quail.